Taking MLST to the Next Level: Microbial Surveillance using Ion PGM™ Sequencing and SeqSphere+ Analysis for Microbial Research.

June 6, 2013 at 10am PST

Dag Harmsen
For public health institutes, animal health labs, epidemiologists and microbiologists interested in the latest improvements in bacterial characterization, this webinar presents recent data and analysis methods using whole genome sequencing. Current methods of microbial surveillance include Multilocus Sequence Typing (MLST), which characterizes isolates of bacterial species using the sequences of internal fragments of (usually) seven house-keeping genes. In this presentation, Professor Harmsen will show how standardized analysis of bacterial assemblies from whole genome shotgun sequencing using the Ion PGM™ system can be achieved in the future for surveillance purposes with a much higher discriminatory power. Methods include the use of Torrent Server Suite Software for bacterial reference mapping (TMAP); de novo assembly (plug-in and MIRA version 3.9) and Ridom SeqSphere+ software for bacterial characterization.

Webinar Details

Your presenters for this webinar are:

  • Prof Dag Harmsen, Head of Research, Center for Dental, Oral and Maxillofacial Surgery Department of Periodontology, University Hospital Münster and CEO, Ridom
  • Dr Chrynsanthi Ainali, Bioinformatics, Life Technologies

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