Bacterial Community Shift in Treated Periodontitis Patients Revealed by Ion Torrent 16S rRNA Gene Amplicon Sequencing

Sebastian Jünemann, Karola Prior, Rafael Szczepanowski, Inga Harks, Benjamin Ehmke, Alexander Goesmann, Jens Stoye, and Dag Harmsen

Department for Periodontology, University of Münster, Münster, Germany and Institute for Bioinformatics, Center for Biotechnology, Bielefeld University, Bielefeld, Germany

Periodontitis, one of the most common diseases in the world, is caused by a mixture of pathogenic bacteria and inflammatory host responses and often treated by antimicrobials as an adjunct to scaling and root planing (SRP). This study aims to elucidate explorative and descriptive temporal shifts in bacterial communities between patients treated by SRP alone versus SRP plus antibiotics. This is the first metagenomic study using an Ion Torrent Personal Genome Machine (PGM).


Jünemann S, Prior K, Szczepanowski R, Harks I, Ehmke B, Goesmann A, Stoye J, Harmsen D (2012) Bacterial community shift in treated periodontitis patients revealed by Ion Torrent 16S rRNA gene amplicon sequencing. PLoS ONE 7(8): e41606. doi: 10.1371/journal.pone.0041606