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Precision TALS fused to Fok1 nuclease Double-stranded breaks can be created at a customer-specified locus in the genome, using a pair of Precision TALs fused to Fok1 nuclease (Figure 1). Using a pair of Precision TALs for the targeting reduces off-target effects. Double-strand breaks are repaired through two mechanisms, homologous recombination or nonhomologous end joining. This repair process is used to perform targeting or gene silencing at any locus. |
Figure 1 |
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